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METHOD AND DEVICE FOR BRIQUETTE PRODUCTION EP13713847.5
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PROCESS FOR REDUCING THE ACRYLAMIDE CONTENT OF HEAT-TREATED FOODS EP03772318.6
[SOYK���ċs��ض'Zk_����D\_�D��UQ03���Ҕ�X��+�mc��4P^.6|���M�$�!��k�� �\����ū�n.^N�OЏw�~pۊ�C�'y�v����ֹߌ����x&-)�5n��^nZ>�,8a�LZx������JKn�s�좓^|��h7IH��S���h$ ���ʀ�|b��`̈́� �T@e�(|w�zh՘���� �y�ߓ7"���rCΨ� ޕ����Ǹ��C����G����d�����͚?, ��]b�"��qu��M=��36bp������ż�a������Ĕ�g�³1]XX[���aMa�r��p��-�R��ç�H86p-�/B��e�\�1���BCS02-5001-EP R9_W�k�`Alfred-Nobel-m���ße 50���)G�D�9tl40789 Monheim am Rhei��*&�kkD�(�\T����R� �Λ�h�3�M�a�z^PS�h4^PS�h4��@� �,e�a, Andrea�K�o����sfx>et alK�ofx>9iid��6117261301201005101@� �J.R. Simplot Company"@P��9iid��61690866@� �Cooley (UK) LLP"@P��9iid��61544956@/�h81>6�>20050608LV20050608MK20050608EP2003012476� num>20031107enWO20040409992004052120042120050817200533 The present invention relates to a process for reducing the acrylamide content of heat-��eated foods, compared with conventional heat-��eated foods. Recently, the Swedish National Food Administration (NFA) and sjvY�tists from Stockholm University have published new research results according to which, in various foods which are given a high heat ��eatment on preparation, acrylamide, a toxic and possibly carcinogenic substa��(92Pi��T&�.HK\�֜�#>���stΏ{�����, �����H��i�3&%'3�\|4� �O�= u��:�aM?�=~�`�!��3���b�k�^Q[���(�7���b�K�����o��x�b9���5����y�͎����/z�g�:v�񋳃 w���v~�o:!L�����#H9�۰!�w�O�G^�����-�f̈́��j ��b��Ge�?�A��|9qv�q��b^��0,�����ag�'�������eM]pG���y�^���gY>V��T���Rk4{t��� �:"�tt��p���ʶ8�G�V� k� F��B�1q7�@�${�f4V7j/^). The X"�U�{W/xL�%9�q��discusse�G��ϔf� towy�g_as esse�ti&�Tend po��s�m��/ִ�toxicologica�Teffec�s�m��q�F��F� q�: neurotoxicity, reproductive toxicity, mutagenicity , ��Gcarcinogenicity. In pdMc�`�ar, the X"�U�{W/xL�%9�q��started from the position that �he genotoxic pote�ti&�Tm��q�F��F� q�>�t7��s�met|V1lic product glycidF� q�><��y�� { importa�t role. In vivo, acrylamideg��pgenotoxic ��5o����c cells>�t7�pgerm cells. I�{Wan thereX�e c�=�e inherit|Vle dF�age 9���T�f�>�hד4`nA�l�3��:۽��9l�]`~���5����*���Z���-�"��@�3p��7M]�ڪ�q���fnEO�7�y�9\{�tAtQ�ur0!� `��De��-D^Z����b'$%����(7A�:�o��h��p���z��{����2�ٽ�������Q�?��u��ۿ�{�3�8�ث6i��Z��ά��9��d�d���u�k��8����G=�p ����ǥ�*��w�|�2o� [�C���VcO_���/��>}4���w��Z�bI����K�Uv�L� �)22�d@RS��e���}oy��GՐ�?�Ic�O�K=s�k}k��7iG�u��2�z`�d����z�ov9r�Ґ��iO���o�O���?:ӷ�\�����,��> ��!��was�m��/ִ�opinion that �o date��carce���ny ev q��cJB� ��Qj�p�6v q�a�����@�iw�\xugړl�R�������mecBjisms,�����example of a ���m\xugڨ��+vV. The :w�R���ӗd&�TX"�U�{W/xL�%9�q��describes the carcinogenic pote�cyTm��q�F��F� q� in [��s_as compar|Vle to that m���o�gcarcinogenic substa��(s�mccurring in c�U�ain foods�G��pdMc�depending on preparation, ����example benzopyrene. Howz� r, acrylamideg��pthe said to mccur ��high�gcontent�pth����H���o�gcarcinogenic substa��(s�found �o date in foods. For humans, the relat����pote�cyTm��carcinogenic substa��(s�in foodsg��pun�-�". The ��>}4���w�epidemiologica�T�Z�bI���f workers X"oO�K=��work are �f lc�O�r��2�z`�d�����9��they are 9r���H��suit|Vle ����de�Rmining sm�H��cBjg(s�in the riskTm��ca9�r. O� r�H�, the X"�U�{W/xL�%9�q��w�|�2o� [�C�presen����z�acrylamide in foods_as c�=�ing W/x�rn�6i��Z��ά�69��d�d���u6">On the bases�m��/ִٽ�VcO_���/��>}, the ��\/�X� X"�U�{W/xL�%9�q��came to th���}oclusion that foods_make� { importa�t contribuq��Lj X"oOure �f W/xL�O�s. Acrylamide is formed when c�U�ain foods are prepar�K=��relat���high tem"�U��+vVs. In @�iw�\x to th��high tem"�U��+vV, the durr^��j�m��X"oOure to high tem"�U��+vVs><��y�� �pdMc. The :w�R���ӗd&�TX"�U�{W/xL�%9�q��did 9r��fi۽��ny �o�greli_���/ev q��cJB����the mecBjism�X������. The O���o�O���?:q�F��F� qɧX������, according to the X"�U�{W/xL�%9�q�, are ��jH��9r��und��'�ood. Und�� c�U�ain X"�Uiment&�T��n�iw�\xs, acrylamidegapp_+*s to Cz��in vitro�in the reaction ?:qmino aAtQs�G��pdMc�`�ar_asparagi�3pVLs:�OS�n9S���672�����]�Ǻsi��T�E��Mottraw�e���H.,�o'�ure 419, (2002), 448, VLs:�OS�n9S���673�����]�Ǻsi��T�E��Stadl�� e���H.,�o'�ure 419, (2002), 449)��2�sug+*s, ����example fr�zose, ga���ose, ���ose��sucrose�VLs:�OS�n9S���674�����]�Ǻsi��T�E��Stadl�� e���H.,�o'�ure 419, (2002), 449). The :w�R���ӗd&�TX"�U�{W/xL�%9�q��7�F���D��0��*1�j�X� , ��G��ϔ��\��{�ommended study�m��/ִ�relat�/xLhip betwQj�p�6cessing W/x�iw�\xs�m��foods an�G��ϔf������� 9f acrylamide, a۽��9l�]`~op�jUbr^��j�m���6cessing W/x�iw�\xs��2�/ִٽim�m��minimizy�g_acrylamide contents. P6cesses for minimizy�g_acrylamide contents�in heat-��eated foods have 9r��ye��Qj�pdescribed �o date in [�C�pri���U�{a۽��re urgY�t��requ��{d. The obj{� thereX�e und��lying the present invention is to �6v qɨ�6cesses which pRmit the producti�j�m��heat-��eated foods which, compared with conventional heat-��eated foods, have a reduc�K=�crylamide content. Th��p�9j{� > ��chieved 0��*1�j�6v s��j�m��/ִ�embodimentspdescribed in [�C�patent cl�ims. The present invention thereX�e relates to a process for reducing the acrylamide content of heat-��eated foods compared with corresponding conventional heat-��eated foods comprising : (a) sel{�ing ��,�tDe��-Dmodified <��nt ����Ui&�Tmrigi�2y�g_1��w�potato <��ntsA�:erein [�C���,�tDeDmodification leads to a reduction in th���}otent of solu���/sug+*s, compared with corresponding non-��,�tDe��-Dmodified <��nt ����Ui&�T1��w�wild ��]� <��ntsA��!��w:erein said ��,�tDeDmodification leads to a reduction in th������b�?��u��@�3p�r mX�e endogenous R1 proteins�mccurring in [�C�p��nt cell compared with corresponding p��nt cell���f wild ��]� <��nts-&�5��� ve 9r��Qj�p��,�tDe��-Dmodified, (b)��6cessing the said <��nt ����Ui&�Tto give a food, ��!� (c) heat-��eating the food produced in process step b)A�:erein [�C�food > �heat-��eated 0��grjH�ing, roasting, extruding, baking or microw ve ho2y�g. Acrylamide VLs:�OS�n9S���676�����]�Ǻc">CAS number 79-06-1)A�:�o> ��9l�e��ed 2-propenamide, vinylamide ���ethylenecarboxamide, > �� solid colourlc�O=��room tem"�U��+vV�:�o> �� ry solu���/in w���U bu/insolu���/in heptane. The �Rm "reduction in acrylamide content", in th���}otext�m��/ִ�present invention, is to be �akex to mean the reducti�j�m��the acrylamide content 0����least 15%�G��pdMc�`�ar_0����least 30%�Gprefer|Vly_0����least 50%�G75% and pdMc�`�arly_prefer|Vly_0����least 90%�Gcompared with the acrylamide content of corresponding conventional heat-��eated foods. Methods of de�Rmining the acrylamide content of foods have �Qj�pdescribed, ����example�G��VLs:�OS�n9S���677�����]�Ǻsi��T�E��Tareke�e���H. (J.�ʹ�s. Food Chem. 50, (2002), 4998-576). The acrylamide was�de�Rmined qu�ntitat���0��GC/MS after d��ivat��a�ln Fe.g. to Cz��the dib��5 product), or_0��LC/MS-MS�Gprefer|Vly_0��LC/MS-MS aspdescribed 0��VLs:�OS�n9S���678�����]�Ǻsi��T�E��Tareke�e���H. (J.�ʹ�s. Food Chem. 50, (2002), 4998-576). D��ivat��a�ln to Cz��the dib��5 producto��h�be carried out, ����example�Gas�in EPA�ڪhod 8032A (http://www.epa.gov/epaoswer/hazwaste/test/pdfs/8032a.pdf, D�cember 1996 version, "Acrylamide 0��gas c��atography")�m��/ִٝS Environment&�TProtecti�j�Age�cyT(= EPA). In th���}otext�m��/ִ�present inventionthe d��ivat��a�ln is prefer|Vly_carried out according to EPA�ڪhod 8032A. m��> 100°C�Gprefer|Vly_m��110°C=to 230°C�G��pdMc�`�ar_120°C-200°C�Gprefer|Vly_m��150°C-170°C�GpdMc�`�arly_prefer|Vle�150°C-180°C. The �Rm "heat ��eatment", in th���}otext�m��/ִ�present invention, is to be �akex to mean �ny ��eatment which, und�� standard press+vV���n�iw�\xs, leads to tem"�U��+vVs>m��above 100°C�Git is to be �akex to mean grjH�ing, roasting, extruding, baking or microw ve ho2y�g, Prefer|Vly, the moist+vV���ntent is�de�Rmined aspdescribed in Iw�R���ӗd&�Tjatent Application WO 97/40707 A1 on page 14. In th���}otext�m��/ִ�present inventionthe English �Rms "potato crisp" and "potato chips" are used instead�m��/ִ�synonymous Am�UiWan tRms "potato chips" and "Fre�ch_1�y". --> ��O�, ��now surprisingly_0eenaCzund �hat �he choi����z�the stdMc�5g <��nt ����Ui&�T1��w�potato <��nts :�o��puO�K=to produce heat-��eated foods ha��a c�Dca�Teffec� on the acrylamide content of suofoods. The inventionte]m�sB����the fi�'� timeo�hat �he =�e m�����nt ����Ui&� :�, compared with corresponding conventional p��nt ����Ui&�, h, ��a reduc�K=�}otent of solu���/sug+*s and/���mino aAtQs pRmits the producti�j�m��foods which, after heat ��eatment, have a lower acrylamide content th���O����CФ�s���z�the =�e m�����nt ����Ui&� having conventional contents�of solu���/sug+*s and/���mino aAtQs. The present invention thereX�e te]m�s,�to avoi�G��ϔf������� 9f acrylamide in heat-��eated foods,�to =�e <��nt ����Ui&� :�o�, ��a compar|t���low=�}otent of solu���/sug+*s and/���mino aAtQs. Methods of de�Rmining the content of sug+*s, ��pdMc�`�ar_fr�zose and�glucose, in p��nt ����Ui&� are �-�" to those ski�ed in th���U�{a۽��re described, ����example�G��VLs:�OS�n9S���67109�����]�Ǻsi��T�E��Mü�er-Röb�� e���H. (Mol. Gen. Genet. 224, (1990), 136-146) a۽��9l�in th��text�:�of� tows. In �}otext�with the present invention, the d��Rminr^��j�m��/ִ�content of glucose, fr�zose and/���sucrose�is prefer|Vly_pRformed aspdescribed 0elow=("D��Rminr^��j�m��glucose, fr�zose and�sucrose"). Methods of de�Rmining the content of qmino aAtQs�G��pdMc�`�ar_asparagi�3, in p��nt ����Ui&� are �-�" to those ski�ed in th���U�{a۽��re described, ����example�G��VLs:�OS�n9S���671 �����]�Ǻ�i��T�E��Co�E�Meys�GTarvin (1988), The pico-tag ڪhod: A Manu&�Tm��qdva9�d techniqu�sB����qmino aAtQ analysis�MjH�ipX�e Corporation, MjH���d�*qdss., USA. Preferred ��pthe ڪhod described 0��VLs:�OS�n9S���6712�����]�Ǻsi��T�E��Roessn�� e���H. (P��nt Physiology 127, (2001), 749-764). The p��nt ����Ui&� used is carac��Uized in that itg��pgen�tDe��-Dmodified, [�C���,�tDeDmodification leady�g_to a reduction in th���}otent of solu���/sug+*s, ��pdMc�`�ar_glucose�and/���fr�zose, compared with corresponding conventional p��nt ����Ui&ꨀf wild ��]� <��nts. ), VLs:�OS�n9S���6714�����]�Ǻsi��T�E��Ritte e���H.,�(PNAS 99, (2002), 7166-7171) a۽�in th��:w�R���ӗd&�Tpatent applications WO98/27212, WO00/77229, WO00/28052 and h ve the carac��Uistics 0elow. Importa�t carac��Uistics m��R1 proteins��re i) [�Cir qmino aAtQ sequence (see, ����example�GGenBank Acc. No. A61831, Y09533), ii) [�Cir loe���a�ln in [�C�p��stids m�����nt cell�, iii) [�Cir qbil�?��to affec� the d�gree m���hos�horyla^��j�m��/ִ�starch in p��nts. Further, th��teRm "R1 protein" refers to a protein catalysing the phos�horyla^��j�m��starch in a dikinrse��]� reaction in :�othree substratesA��! α-polyglucan�GATP and H2O��re converted into three fnEO�7�yA��! α-polyglucan-P�GAMP� {��׃�hophos�hate VLs:�OS�n9S���6715�����]�Ǻsi��T�E��Ritte e���H.,�PNAS Vol. 99 No. 10, (2002), 7166-7171). A�synonymA�:�o> used in the mo�e recent l�?�U��+vV�����t�8��Rm "R1 protein", is�t�8��Rm "GWD protein" :�o��pth���bbrevia^��j�����"al�ha-glucan w���U dikinrse" VLs:�OS�n9S���6716�����]�Ǻsi��T�E��Blennow e���H.,�Trends�in P��nt �jvY��("Vol. 7 No. 10 (2002), 445-450). ThereX�e, with rs�k}t to the present invention, the teRm "R1 protein" comprisesG�9l�"GWD proteins". F���example�G�hibiting the R1 ��,� codingB����qn�R1 proteinT1��w�potatoesGleads, in transgenic potato <��ntsA�to a reduction in th��phos�hate �}otent of /ִ�starch :�o��h�be�isolated from the potato tubers. MX�eo� r, Lorb��th e���H. show �hat �he R1 proteinT1��w�Sola�d� tuberosum > ��ble to phos�horyla^e bac��Ui&ꨮtAogen when th���}rresponding R1 cٽ�). VLs:�OS�n9S���6718�����]�Ǻsi��T�E��Ritte e���H. (P��nt J. 21, (2000), 387-391) showed �hat �he R1 proteinT1��w�Sola�d� tuberosum >n�potato <��nts reversiVly_0inds to starch granules,�with the �Icngth of /ִ�0inding to the starch granule�being dependent on the ڪ�q���status�m��/ִ�p��nt. In th��Cz��bound �o starch granules,�the proteinT>n�potato <��nts is priS��p��-Dpresent in�leaves-&�5��� ve �Qj�p�r�" in th��dark. After jH�umi�2y�g_�T�faves-the protein, in contr�st, is priS��p��-Dpresent in�the solu���/Cz��:�o��p9r��ound �o the starch granule. ), that is to say cold-stored potato tubers�Gcompared with tubers m��corresponding wild ��]� <��nts which are 9r��gen�tDe��-Dmodified, have a reduc�K=�}otent of solu���/sug+*s, ��pdMc�`�ar_fr�zose and�glucose. In @�iw�\x, the potato tubers�m��/ִse transgenic p��nts having reduc�K=R1 ��,� X"Ic�O��j, even immediate��after harvest, or_after storage 9��room tem"�U��+vV�Gcompared with tubers m��corresponding wild ��]� <��nts which are 9r��gen�tDe��-Dmodified, have a reduc�K=�}otent of solu���/sug+*s, ��pdMc�`�ar_fr�zose and�glucose. ), leads to a markedly_reduc�K=�crylamide formation in the d�ep-fat-fried fnEO�7�y�th���when corresponding potato tubers�which � ve 9r��Qj�p��,�tDe��-Dmodified��re de9�7N�݊�p���QT?�ft4�), U2�G6�\t�$���d��Ϊ��6���p5�^uv��|�M{�R�#���N�.?�8?�� [&��)�H�M�V�H��"�k����sC�� �^�L՘4�$N�e�\ĴI�����'ɧ��~̔ ��և�J"�FB�����I C�b��m��d�"�J�d���%4n�a� 1��|��A(�&|e���9���_��q l.�x@�, ��b2�% ��Ȑs@���d���"N-'����3����I��f�9�y+X,Ƶ��Ì&�&, ���������J�Q������hF@��FzXN7��HZ�+�J��֪�ױ�� ��vǂc�X�0&4�0���w�ܔ���y/n #j�['�Y�1�!b��t��T�W�5N⬩'�� v�)�� v\C�)^b��Y��#�z�VhQ�҇���יS�c��,6����T��U!�_dA�Ҫ��H��9UY�� ����`��YI�"�0N {�� > n�th���ell���f [�C�p��nt ����Ui&�T�nd/��� reducti�j�in th��Xnzy����c ����b�?��u��he R1 proteinsT>n�th���ell���f [�C�p��nt ����Ui&� compared with corresponding non ��,�tDe��--modified cell���f wild��]� <��nts. A reducti�j�in th��Xnzy����c ����b�?��u��he R1 protein�prefer|Vly_means a reduction in ����b�?��compared with corresponding non ��,�tDe��--modified cell��0����least 50%�G��pdMc�`�ar_0����least 60%�Gand prefer|Vly_0����least 70%. A reducti�j�in th��Xnzy����c ����b�?��u��he R1 protein�prefer|Vly_means a seduction in ����b�?��m��R1 compared with R1 ����b�?��m��corresponding non ��,�tDe��--modified cell��0����least 50%�G��pdMc�`�ar_0����least 60%�Gand prefer|Vly_0����least 70%. n�the sk}kf��spati&� arrjg(ment�ur:�o��ploe���ed at a site in [�C�g���o��f [�C�p��nt cell at�:�o�Odoes 9r����+v��-Dmccur. Prefer|Vly, the X�eign��dcle� aAtQ mXlecule > ����{�ombin�nt mXlecule-&�5���}osists m��various el(ments,�the �ombin�ti�j�mr sk}kf��spati&� arrjg(ment�uf_:�odoes 9r��mccur ���+v��-Din p��nt cell�. (a) ٽ� (b)�ٽ� (c) ٽ� (d)��dcle� aAtQ mXlecules which are introduced 0��means ��7n vivo mutagen��> ��!��lead to a mutati�j�mr insertion of a he�Rologous sequence in �l�3��codingB����endogenous R1 proteins, the mutati�j�mr insertion c�=�ing a reducti�j�in the X"Ic�O��j�m��/ִ�said ��,�s ���t�8�syn�hesis ��7n����b'$R1 proteins, (e) ٽ� (f) ٽ� (g) T-ٽ� n�th���ell�. Suit|Vle sequences are g�,�v��-Dsequences up�to a minimum length of 15 bp, prefer|Vly_a minimum length of 21 bp, prefer|Vly_a length of 100-57 bp, bn�aCz��qn�XffijvY�t�antisense or cosup"Ic�O��j�inhibition,��dMc�`�ar_preference is given to sequences having a length ovRR�57 bpN�H�~se stat(ments apply correspondingly_to the :whibition of BE I���,� X"Ic�O��j. ��9l�e/x�iv|Vle, that is to say non-coding regions of �l�3��:�o�}de for�qn�R1 proteinTprotein. The =�e m��intron�sequences fmr inhibiting ��,� X"Ic�O��j�of �l�3��:�o�}de for�proteins�m��starch biosyn�hesis �, ���W��described, ����example�G��th��:w�R���ӗd&�Tpatent applications WO97/04112, WO97/04113, WO98/37213, WO98/37214N�H�~se stat(ments apply correspondingly_to the :whibition of BE I���,� X"Ic�O��j. A_pRson ski�ed in th���U�{is familiar_with how �o��chieve an�antisense , ��Gcosup"Ic�O��j�effec�. The process m��cosup"Ic�O��j�inhibition �, ���W��described, ����example�G��VLs:�OS�n9S���6722�����]�Ǻsi��T�E��JorgY�sen (Trends�Biotechnol. 8 (1990), 340-344), VLs:�OS�n9S���6723�����]�Ǻsi��T�E��Niebel e���H.,�(Curr. TopN�Microbiol. Immunol. 197 (1995), 91-103), VLs:�OS�n9S���6724�����]�Ǻsi��T�E��Fl vell e���H. (Curr. TopN�Microbiol. Immunol. 197 (1995), 43-46), VLs:�OS�n9S���6725�����]�Ǻsi��T�E��Palaqui , ��GVau]m��e��(P��nt. MXl. Biol. 29 (1995), 149-159), VLs:�OS�n9S���6726�����]�Ǻsi��T�E��Vau]m��e��e���H.,�(Mol. Gen. Genet. 248 (1995), 311-317), de VLs:�OS�n9S���6727�����]�Ǻsi��T�E��Borne e���H. (Mol. Gen. Genet. 243 (1994), 613-621). EP-B1 0321201N�H�~���Ic�O��j�of ribozy�es in p��nt cell� �, ���W��described, ����example�G��VLs:�OS�n9S���6728�����]�Ǻsi��T�E��Feyt�� e���H. (Mol. Gen. Genet. 250, (1996), 329-338). In @�iw�\x, the reducti�j�m��the R1 ����b�?��in th��p��nt cell���f [�C�p��nt ����Ui&� c����H9l�be��chieved 0��"in-vivo mutagen��> ", in :�, 0��*ransf������� 9f cell�, a hybrid R��-ٽ�, VLs:�OS�n9S���6730�����]�Ǻ�i��T�E��R. A. DixY|���HC.J.��rntz�E�Meeting re�z` on "Mڪ�q���Engi�3ering in Transgenic P��nt ", KeystoneGSymposia,TCoppR MXuotain,TCO, USA, TIBTECH 15, (1997), 441-447, :w�R���ӗd&�Tpatent application�Vpat:�OS�n9p���67109�d�d�d�WO9515972A">WO 9515972, VLs:�OS�n9S���6731�����]�Ǻsi��T�E��Kr��X���H.,�Hepatology 25, (1997), 1462-1468, VLs:�OS�n9S���6732�����]�Ǻsi��T�E��CXle-S*rauss X���H.,ĨjvY��("273, (1996), 1386-1389, VLs:�OS�n9S���6733�����]�Ǻsi��T�E��Beethaw�e���H.,�(1999),�PNAS 96, 8774-8778). A_p�U�{m��/ִ�ٽ�<�}mponent�u��he R��-ٽ� By base-pairing the �omologous regions�u��he R��-ٽ� --> f� tow�K=0���omologous re�ombin�ti�j, the mutati�j�mr he�Rologous regionpresent in�the ٽ�<�}mponent�u��he R��-ٽ� In @�iw�\x, the R1 ����b�?��c����H9l�be�reduc�K=in th��p��nt cell��0��*1�jsjU��tane�S���Ic�O��j�of sense , ��Gantisense R�� Th��p��h�beٙchieved, ����example�Gby =�ing Whimer�c constr�7�y�:�o�}otain�inverted repeats�m��/ִ�re�k}tive targY��gen� or pdM�s�m��/ִ�targY��gen�. In th��p��se the cimer�c constr�7�y��}de for�sense , ��Gantisense R��in p��nta sjU��tane�S��as�mne R�� ��9l�e��ed "R��i technology". ��O�, ���W��Xh�" that itroducy�g_inverted repeat ٽ�<�}nstr�7�y��nto the g���o��f <��nts is a highly effijvY�t�ڪhod for re�Ic�O�ng the �l�3��corresponding to the inverted repeat ٽ�<�}nstr�7�y�VLs:�OS�n9S���6734�����]�Ǻsi��T�E��W���Uho=�e e���H.,�Proc. NatlN�Acad.Ĩjv. USA 95, (1998), 13959-13964, VLs:�OS�n9S���6735�����]�Ǻsi��T�E��W�ng ۜ�GW���Uho=�e,�P��nt MXl. Biol. 43, (2000), 67-82, VLs:�OS�n9S���6736�����]�Ǻsi��T�E��Singh e���H.,�Bio`��De� SojvY?��Transactions"Vol. 28_p�U�{6 (2000), 925- 927, VLs:�OS�n9S���6737�����]�Ǻsi��T�E��Liu e���H.,�Bio`��De� SojvY?��Transactions"Vol. 28_p�U�{6 (2000), 927-929), VLs:�OS�n9S���6738�����]�Ǻsi��T�E��Smith e���H.,�(o'�ure 407, (2000), 319-320, :w�R���ӗd&�Tpatent application�Vpat:�OS�n9p���671 ��d�d�d�WO9953050A1">WO99/53050 A1). Sense , ��Gantisense sequences m��/ִ�targY��gen� ���t�8�targY��gen�s�c����H9l�be�X"Ic�O�K=separately_�rom oneGan�o�g0��means ��th��same ���different p��5ters�VLs:�OS�n9S���6739�����]�Ǻsi��T�E��Nap, J-P e���H, 6th Iw�R���ӗd&�TCo�gress m��P��nt MXlecular_Biology, Quebec, 18-24 June,\�#�0, Poster S7-27, Presentation�Sc�O��j�S7).�H�~se stat(ments apply correspondingly_to the :whibition of BE I���,� X"Ic�O��j. in �rans c���lead to a methyl�q��w��Ga�transcriptӗd&�T7n����b���� 9f �omologous copies�u��hese pro�5ters�which are to be �ermed :ereinafter�targY��p��5ters�VLs:�OS�n9S���67409�����]�Ǻsi��T�E��Mette e���H.,�EMBO J. 19, (2000), 5194-5201). Via the :w����b���� 9f t�8�targY��p��5ter�it is thus�possible to reduc� [�C���,� X"Ic�O��j��f a�c�U�ain targY��gen� (����example R1 gen�)-&�5��exists ���+v��-Dund�� th���}otRol��f this targY��p��5ter. That is to say the ٽ� To produce the dou���-stranded targY��p��5ter�R��in p��nta :�o��h�be�present there as R��in p��nta to the f������� 9f dou���-stranded targY��p��5ter�R��). By th��pmeans /ִ�targY��p��5ter���h�be�iw����b��{d. The reducti�j�in R1 ����b�?��in th��p��nt cell��c���thus��H9l�be��chieved 0��producy�g_dou���-stranded R�� �given ��VLs:�OS�n9S���6742�����]�Ǻsi��T�E��Ram�chandra��w��GSundaresa��(2001,�P��nt Physiology w��GBio`��Dstry 39, 234-252). The possibil�?��?:identifying mutants in :�osk}kf�⑄l�3��� ve �Qj�piw����b��{d 0��*ransposon O�sertion mutagen��> �ispdescribed in a�review�0��VLs:�OS�n9S���6743�����]�Ǻsi��T�E��Maes e���H. (1999,�Trends�in P��nt �jvY��("4 (3), 90-96). Producy�g_ri���mutants w2�/ִٽid�m��Xndogenous transposons���pdescribed 0��VLs:�OS�n9S���6744�����]�Ǻsi��T�E��Hi6chika�(2001,�Current Opini�j�in P��nt Biology 4, 118-122). The identification�m��maizC�gen�s�=�ing Xndogenous retRotransposons���pre�z`ed, ����example�Gby VLs:�OS�n9S���6745�����]�Ǻsi��T�E��Hanley e���H. (2000, The P��nt Jour�l 22 (4), 557-566). The possibil�?��?:producy�g_mutants =�ing retRotransposons�w��Gڪhods fmr identifying mutants �re describedGby VLs:�OS�n9S���6746�����]�Ǻsi��T�E��Kumar a��GHi6chika�(2001,�Trends�in P��nt �jvY��("6 (3), 127-134). The ac��b�?��u��he�Rologous transposons�in�different sk}ke� �, ���W��described 9r��mnly_f���dicotyledonous bu/aH9l�for monocotyledonous <��nts: e.g. for ri���VLs:�OS�n9S���6747�����]�Ǻsi��T�E��Gre�o e���H.,�2001,�P��nt Physiology 125, 1175-1177, VLs:�OS�n9S���6748�����]�Ǻsi��T�E��Liu e���H.,�1999,�MXlecular_a��GG�,�v� Genetics 262, 413-420, VLs:�OS�n9S���6749�����]�Ǻsi��T�E��Hi6yuki e���H.,�1999,�The P��nt Jour�l 19 (5), 605-613, VLs:�OS�n9S���67509�����]�Ǻsi��T�E��Jeq��w��GGynheung, 2001,�P��nt �jvY��("161, 211-219), barley VLs:�OS�n9S���67519�����]�Ǻsi��T�E��2000, Ko"Ick e���H.,�The P��nt Jour�l 24 (2), 253-263) Ar|Vidopsis thaliana VLs:�OS�n9S���6752�����]�Ǻsi��T�E��AdM�s�e���H.,�1993,�o'�ure 363, 715-717,VLs:�OS�n9S���6753�����]�Ǻsi��T�E�� �jhmid�{a۽�Wi�mitz r, 1989,�MXlecular_a��GG�,�v� Genetics 220, 17-24, VLs:�OS�n9S���6754�����]�Ǻsi��T�E��AltmanѮe���H.,�1992,�TheX�eDca�Ta��GApplied Genetics 84, 371-383, VLs:�OS�n9S���6755�����]�Ǻsi��T�E��Tissi�� e���H.,�1999,�The P��nt Cell 11, 1841-185Q), tomato VLs:�OS�n9S���6756�����]�Ǻsi��T�E��Belzile , ��GYod r, 1992,�The P��nt Jour�l 2 (2), 173-179) a۽�potatoesGVLs:�OS�n9S���6757�����]�Ǻsi��T�E��Frey e���H., 1989,�MXlecular_a��GG�,�v� Genetics 217, 172-177, VLs:�OS�n9S���6758�����]�Ǻsi��T�E��Knapp e���H., 1988,�MXlecular_a��GG�,�v� Genetics 213, 285-290). Agrobac��Uium c���O��gra^e �nto the g���o��f <��nt��ell�. T1�jsjte m��intgra^��j�in th��p��nt ch��5s�o���p9r��fixed :ere, that ��h�beٙt �ny desired position. Iz�the T-ٽ�Ar|Vidopsis thaliana VLs:�OS�n9S���6759�����]�Ǻsi��T�E��Krysa��e���H.,�1999,�The P��nt Cell 11, 2283-2290, VLs:�OS�n9S���67609�����]�Ǻsi��T�E��Atipi6z-Leeha��w��GFeldman�, 1997, Trends�in genetics 13 (4), 152-156, VLs:�OS�n9S���6761�����]�Ǻsi��T�E��Parinov�w��GSundaresa�,\�#�0,�Current Opini�j�in Biotechnology 11, 157-161) a۽�ri���VLs:�OS�n9S���67629�����]�Ǻsi��T�E��Jeq��w��GAn, 2001,�P��nt �jvY��("161, 211-219, VLs:�OS�n9S���67639�����]�Ǻsi��T�E��Jeq��e���H.,�2000, The P��nt Jour�l 22 (6), 561-570). Mڪhods fmr identifying mutants &�5��� ve �Qj�pproduced =�ing T-ٽ� ��re described, :w�R alia�Gby VLs:�OS�n9S���6764�����]�Ǻsi��T�E��Young X���H.,(2001,�P��nt Physiology 125, 513-518), VLs:�OS�n9S���6765�����]�Ǻsi��T�E��Parinov�e���H. (1999,�The P��nt cell 11, 2263-2270), VLs:�OS�n9S���6766�����]�Ǻsi��T�E��Thorneycroft e���H. (2001, Jour�l m��Exp�Uimentl Botany 52, 1593-1601), bn�aVLs:�OS�n9S���6767�����]�Ǻsi��T�E��McKinney e���H. (1995, The P��nt Jour�l 8 (4),613-622). The naming of �he isoCz��s :ere�ispbased on the ��oncl'�ure propoO�K=0��Smith-Whjte bn�aPreiss�VLs:�OS�n9S���6768�����]�Ǻsi��T�E��Smith-Whjte bn�aPreiss,�P��nt MXl Biol. Rep. 12, (1994), 67-71, VLs:�OS�n9S���6769�����]�Ǻsi��T�E��Larssq��e���H.,�P��nt MXl Biol. 37, (1998), 505-511). Th��p9roncl'�ure stdMcs from the position that all enzy�es &�5��� ve a higher homology (identity)�at �he qmino aAtQ level to the BEI protein from maizC�(GenBank Acc. No. D11081, VLs:�OS�n9S���67709�����]�Ǻsi��T�E��Baba e���H.,�Bio`��. Biophys. Res. Commun. 181 (1), (1991), 87-94, VLs:�OS�n9S���6771�����]�Ǻsi��T�E��Kiw�e���H. Gene 216, (1998), 233-243) th���to the BEII protein from maizC�(Genbank Acc. No AF072725, U65948), �re c��ed branching Xnzy�em��isoCz��I, or_for�shz` BEI proteins. ), Genbank Acc. No. Y 08786 a۽�in VLs:�OS�n9S���67739�����]�Ǻsi��T�E��KossmanѮe���H.,�Mol. Gen. Genet. 230, (1991), 39-44). In potato <��ntsA�the BEI ��,� is priS��p��-DX"Ic�O�K=in the tubers�and�scarce��at all in the �faves-VLs:�OS�n9S���6774�����]�Ǻsi��T�E��Larssq��e���H.,�P��nt MXl. Biol. 37, (1998), 505-511). n�th���ell���f [�C�p��nt ����Ui&�T�nd/��� reducti�j�in th��Xnzy����c ����b�?��u��he BEI proteins >n�th���ell���f [�C�p��nt ����Ui&�. --> In a further�preferred embodiment�u��he inventivɨ�6cess, [�C�X�eign��dcle� aAtQ mXlecule :�oleads to the reducti�j�in a���b�?��u��@�3p�r mX�e endogenous BEI proteins m��isoCz��I�mccurring in [�C�p��nt cell > �sel{�ed from the group��}osisting of (a) ٽ� (b)�ٽ� (c) ٽ� (d)��dcle� aAtQ mXlecules which are introduced 0��means ��7n vivo mutagen��> ��!��lead to a mutati�j�mr insertion of a he�Rologous sequence in �l�3��codingB����endogenous BEI proteins,Tthe mutati�j�mr insertion c�=�ing a reducti�j�in the X"Ic�O��j�m��/ִ�said ��,�s ���t�8�syn�hesis ��7n����b'$BEI proteins, (e) ٽ� (f) ٽ� (g) T-ٽ� In a further�embodiment,�/ִ�present invention�rel2esTto the =�e m��th���bove describedBp��nt ����Ui&�, :�o��h�be�used in the inventivɨ�6cess for�producy�g_heat-tre���dB��ods &�5��� ve a reduc�K=�crylamide �}otent compared with corresponding conventӗd&�Theat-tre���dB��ods. The present invention�discloO�sTa��6cess for�identifying p��nt ����Ui&� :�oispsuit|Vle for�producy�g_heat-tre���dB��ods having a reduc�K=�crylamide �}otent, :�o�}mprises: a) de�Rmining th���}otent of solu���/sug+*s �nd/���mino aAtQs in p��nt ����Ui&� :�oispsuit|Vle for�producy�g_heat-tre���dB��ods, ��!� b)�sel{�ing suop��nt ����Ui&�T�ccordy�g_to �6cess step a) :�, compared with corresponding conventӗd&�Tp��nt ����Ui&�, has a reduc�K=�}otent of solu���/sug+*s �nd/���mino aAtQs. Mڪhods: De�Rmination of �lucose,_fr�zose and�surose: 17 mM imidazXle/HCl (pH 6.9) 5 mM MgCl2 2 mM NAD+ 1 mM ATP 27 µl m��sample 2 units of �lucose-6-phosph���dehydrogen�se (from Leuconosto�mesentRoides) The �ssay solu^��j�> �O�cub���dB9��room tem"�U��+vVB����5 Oin. T1�jsug+*s �re then de�Rmined =�ing Wustomary photometr�c ڪhods by measuring the absorpt��j�at 340 nm_after the successivɨ@�iw�\x of 1�57 units of yeast hexokin�se (to de�Rmine �lucose) 2.5 units of yeast phospho�lucois�or�se (to de�Rmine fr�zose) 35 units of yeast β-fr�zosidase (to de�Rmine surose) to a reaction volu�em��27 µl. ComparativɨExample 1 Producti�j�m��potato crisps a۽�potato cipsT1��w�potato tubers ) a۽��9l�potato tubers�m��potato <��nts &�5��� ve a decreased R1 ��,� X"Ic�O��j a۽�in @�iw�\x a decreased X"Ic�O��j�m��branching Xnzy�eI���,� Vpat:�OS�n9p���6712��d�d�d�WO9711188A">WO97/11188) were used. The crisps a۽�cipsTwere further�processedB�mmediate��after harvest a۽��9l�after storageٙt 4°C f���differing times. The samples were deep-fat friu� in a�deep-fat fryer (Frita4, Franke, Frifri aro GmbH, Germany) f���differing times =�ing p��nt fat (Palmaja�Meylip mbH & Co. KG, Germany) at a tem"�U��+vVBof 180°C. In�th���a�e m��all deep-fat friu� samples,�itgwas found that �he ��rylamide �}otent in the transgenic potato �ubers�was reduc�K=0����least 15% compared with the ��rylamide �}otent u��the tubers�m��/ִ�corresponding wild ��]� <��nts. ComparativɨExample 2 De�Rmination of the ��rylamide �}otent u��potato crisps a۽�cipsTproduced 1��w�potato tubers having decreased R1- a۽�branching Xnzy�eI�-���,� X"Ic�O��j. Non-��,�tDe��--modified <��nts �re termed :ereinafter�wild ��]� <��nts. T1�j/ransgenic potato <��nts &�5��� ve a decreased X"Ic�O��j�m��/ִ�R1 ��,� VLs:�OS�n9S���6776�����]�Ǻsi��T�E��Lorb��th e���H.,�o'�ure Biotechnology 16, (1998), 473-477) a۽�in @�iw�\x a decreased branching Xnzy�eI���,� X"Ic�O��j�(see :w�R���ӗd&�Tpatent application�Vpat:�OS�n9p���6713��d�d�d�WO9711188A">WO 97/11188) �re termed :ereinafter�015VL671. If freshly harvested tubers�m��potato <��nts are used f���deep-fat frying at 180°C f���3 a۽�6 minu��s,�the potato crisps � ve /ִ�f� towing �crylamide �}otent: T|Vle�1: Percentageٙ�rylamide �}otent u��crisps (produced 1��w�potato tubers dirct��after harvesting). The wild ��]� was se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="57mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="55mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="55mm"/> Crisps deep-fat frying timeo3 Oin Crisps deep-fat frying timeo6 Oin Wild ��]� 17% 17% 015VL671 31% 49% The �bsolu^e ��rylamide �}otent increases gre� with increasing deep-fat frying time. Th��p��pth���a�e 9r��mnly_f���crisps 1��w�wild ��]� tubers�Gbu/aH9l�for crisps 1��w�/ransgenic tubers. Fz��both deep-fat frying times, the :wcrease�in a�rylamide �}otent in the crisps &�5��were produced 1��w�the transgenic potato �ubers, howev r, ispsignificant��reduc�K=�}mpared with the �risps mf wild ��]� <��nts. At a�deep-fat frying timeomf 3 Oin, �he ��rylamide �}otent in the transgenic �risps is reduc�K=0���pproximate��70% compared with the wild ��]� �risps. At a�deep-fat frying timeomf 6 Oin, �here > ����{ducti�j�in a�rylamide f������� in the transgenic �risps m��approximate��50% compared with the wild ��]�. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="57mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="55mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="55mm"/> Crisps deep-fat frying timeo3 Oin Crisps deep-fat frying timeo6 Oin Wild ��]� 17% 17% 015VL671 26% 28% The �bsolu^e ��rylamide �}otent always gre� increases in [�C�products from potatoesGstor�dB9��4°C. Howev r, it is Xh�" that �he ��rylamide �}otent in the crisps made 1��w�/ransgenic potato tubers increases 0���pproximate��70% lesso�}mpared with the �risps made 1��w�corresponding wild ��]� <��nts,�both for���deep-fat frying timeomf 3 Oin, bn�aCz��q�deep-fat frying timeomf 6 Oin. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="57mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="55mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="55mm"/> Potato cipsTdeep-fat frying timeo3 Oin Potato cipsTdeep-fat frying timeo6 Oin Wild ��]� 17% 17% 015VL671 55% 42% The �bsolu^e ��rylamide �}otents are tow�r in [�C�potato cipsT�}mpared with potato crisps. Th��p��pc�U�ainly_primarily_due_to the smaller surfaceT�rea��f [�C�potato cipsT�}mpared with potato crisps_pR kg u��potato. The percentageٙ�rylamide �}otents, in th��pproduct/aH9l, Xh� a reducti�j�in the potato cipsTmade 1��w�/ransgenic potato <��nts 0���pproximate��50% at both deep-fat frying timesT�}mpared with potato cipsTmade 1��w�wild ��]� tubers. In�th���}otext�m��industri&�Tproducti�j�m��crisps mr potato cips, the sliced potatoesGwere blanch�K=0eX�e deep-fat frying. The blanching W���tak� <��ce in a water or�steam blanch�r. The blanching W}o�iw�\x�pare 9r��fixed values�Gbu/vary v ry gre� depending on the qual�?��u��he potatoesGused. During the blanching, solu���/sug+*s �re par wash�K=mu`. Th��p��=�es�mX�e unifz��browning of �he potato F���this purpose, �pproximate��27 g u��potatoes (stor�dB9��4°C ����56 daysafter harvest) were wash�K=three times, each timeo=�ing 5 l�?res m����ins aterB9��45°C, each timeo����1.5 minu��s. T1�jsliced potatoesGwere /ִn�driu� on�domestic pap r a۽�deep-fat friu� at 180°C f���3 minu��s aspdescribed �bove: T|Vle�4: Percentageٙ�rylamide �}otent u��wash�K=crisps (produced 1��w�potato tubers). The wild ��]� was se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="84mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="82mm"/> Crisps deep-fat frying timeo3 Oin Wild ��]� 17% 015VL671 21% The washing leads to a reduction in a�rylamide f������� in crisps &�5��were produced 1��w�potato tubers�m��wild ��]� <��nts 0���pproximate��16% compared with unwash�K=potato crisps. Compared with the "wash�K" crisps &�5��were produced 1��w�potato tubers�m��wild ��]� <��nts, the "wash�K" crisps &�5��were produced 1��w�potato tubers�m��t1�j/ransgenic potato <��nts � ve an a�rylamide f������� :�oispdecreased b���pproximate��80%. F���this purpose the potato �ubers�were peel�K=w��Ga�sample having a diame�R m��approximate��0.5 cm�sample was �ut out =�ing a �}rk borer (1��w�Roth). F��w�this Xample a�slice �pproximate��2 mw�thick each timeo���w�the stdMc,�oneGquart r a۽�oneGhalfo���w�5�different tubers in each �a�e was �ombin�K=in a reaction vesse�Ta��Gused to de�Rmine solu���/sug+*s. The de�Rmination of the �}otents of sug+*s�G��pdMc�`�ar_fr�zose and�lucose,_m��p��nt ����Ui&� ��p�-�" to those ski�ed in th���U� and�was �arriu� out aspdescribed �bove. T|Vle�6: Comparison of the percentage�solu���/sug+* �}otents of fresh harvested and�stor�dBtuber samples based on tubers�m��/ִ�corresponding wild ��]� (17%). <�}lspec colnum="1" colnam�Ǻcol1" colwidth="27mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="35mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="35mm"/> <�}lspec colnum="4" colnam�Ǻcol4" colwidth="35mm"/> <�}lspec colnum="5" colnam�Ǻcol5" colwidth="35mm"/> Wild ��]� dirct��after harvest 015VL671 dirct��after harvest Wild ��]� after storageٙt 4°C 015VL671 after storageٙt 4°C Glucose [µmol/g fresh weight] 17% 61% 17% 48% Fr�zose [µmol/g fresh weight] 17% 67% 17% 53% SuroseT[µmol/g fresh weight] 17% 110% 17% 69% If th���}otent of �lucose ���fr�zose i��correl���dBwith the ��rylamide �}otent in crisps, it is XQj�pthat �here > ���line+* �}rrel����j�0etwQj�pth���}otent of �lucose ���fr�zose in the potato �uber�and�the f������� 9f ��rylamide in the deep-fat friu� product crisps. ����}rrel����j�0etwQj�pth��f������� 9f ��rylamide in heat-tre���dB��ods and�the �}otent of solu���/sug+*s �f [�C�p��nt ����Ui&�Tused to produce the heat-tre���dB��odN�H�~��ffec� on�reduction of a�rylamide f������� is muomX�e pronounc�K=than X"cted. ComparativɨExample 3 De�Rmination of the ��rylamide �}otent u��potato crisps a۽�potato cipsTproduced 1��w�potato tubers having decreased R1-��,� X"Ic�O��j. In�th��p��se, as already described in example 2, firstly�/ests were made u��differing deep-fat frying times, a۽��9l�samples w�5��� d �Qj�pdifferently�stor�dBurash�K=were studied. The results described in example 2=were �}ofirmed, that is to say potato crisps a۽�potato cipsTproduced 1��w�potato tubers having decreased R1���,� X"Ic�O��j, likewise Xh�, =nd�� th���}o�iw�\x�pdescribed in mXre de�ail in example 2, lesso�crylamide �}mpared with corresponding products w�5��were produced 1��w�potato tubers�from corresponding non-��,�tDe��--modified wild ��]� <��nts. Example 4 Producti�j�m��differing vari�tDes�m��/ransgenic potato <��nts having reduc�K=R1���,� X"Ic�O��j ). No��s on vezor IR5/29: IR5-29 > ���derib���ve) in the "sense" mrientaw�\x to /ִ�pr��5ter. pGSV71�> ���derib���ve ���derib���ve). pGSV1�wa�pobtained 1��w�pGSC1700 0��deletion of the �arb�nicillin resistance ��,� {��deletion of the T-ٽ� pGSV7o�}otains the replication�mrigi�u��p��smid pBR322 (VLs:�OS�n9S���67809�����]�Ǻsi��T�E��Boliv+* e���H., (1977), C}ostr�7���j�a۽�carac��Uization of new cloning ve:�les.�II. A�U��tipurpose cloning syst�. Gene,�2: 95-113) a۽�the replication�mrigi�u��/ִ�pseud��5nas�p��smid pVS1 (VLs:�OS�n9S���6781�����]�Ǻsi��T�E��Itoh e���H., (1984), Gen3tDeDw��GXlecular_carac��Uization of /ִ�Pseud��5nas�p��smid pVS1.�P��smid 11: 206-220). pGSV7o�9l��}otains the sel{�|Vle�marker���,� adA 1��w�the transposon Tn1331 1��w�Klebsiella pneu�5niae, :�o�}nfers�resistance to /ִ�antibiotics spectinomycin and�streptomycin (VLs:�OS�n9S���67829�����]�Ǻsi��T�E��Tolmasky, (1990), Sequencing and X"Ic�O��j�m�� adA, bla, a۽�tnpR 1��w�the U��tiresistance transposon Tn1331.�P��smid. 24(3): 218-226, VLs:�OS�n9S���67839�����]�Ǻsi��T�E��Tolmasky a۽�Crosa, (1993), Gen3tDeDorganization of antibiotic resistance ��,�s (aac(6')-Ib,� adA, a۽�oxa9) in the U��tiresistance transposon Tn1331.�P��smid. 29 (1): 31-40). T�C�p��smid pGSV71�a�pobtained 0��cloning a chimer�c bar���,� 0etwQj�pth��border), th��bar���,� 1��w�StreptomycesG�ygroscopicus-VLs:�OS�n9S���6785�����]�Ǻsi��T�E��Thompsq��e���H.,�(1987), Carac��Uization of /ִ�herbicide resistance ��,� bar_fr�w�StreptomycesG�ygroscopicus. The EMBO Jour�l,�6: 2519-2523) a۽�the untransl���dB3' T�C�T-ٽ�<�}otains the f� towing el{ments in the order the �fft�border). /ִ�pr��5ter�m��/ִ�patatin �l�3 B33 1��w�Solanum tuberosum (Rocha-Sosa e���H., 1989,�see �bove) in a�sense mrientaw�\x based on the �fft�border /ִ��}mplet��R1-cٽ�<(Lorb��th e���H.,�(1998), see �bove) in a�sense mrientaw�\x based on the patanin pr��5ter /ִ�polyadenyl����jpsign&�T(3' /ִ�Taql�fragment�u��he non-transl���dB3') in a��w�tisense mrientaw�\x based on the �fft�border /ִ��}dy�g_sequence m��/ִ�phosphinothricin resistance ��,� (bar) 1��w�StreptomycesG�ygroscopicus-Thompsq��e���H.�(1987, see �bove) in a��w�tisense mrientaw�\x based on the �fft�border /ִ�P35S3�pr��5ter�regionu��cauliftow�r mosa�c virus (Odell e���H., (1985), see �bove) in a��w�tisense mrientaw�\x based on the �fft�border /ִ�right�border Potato tubers�from lines 093IR360, 095IR049 a۽�092IR002 were used to produce potato cipsT(example 5). ComparativɨExample 5 De�Rmination of the ��rylamide �}otent u��potato cipsTw�5��� d �Qj�pproduced 1��w�different vari�tDes�m��potato tubers having decreased R1���,� X"Ic�O��j Freshly harvested potato tubers�m��t1�j<��nts produced �ccordy�g_to example 4�were processedBto potato cips in a�cordance with example 1 a۽�"Ic-deep-fat friu� accordy�g_to example 2 f���30 seconds�at 180°C, l�id�mu` on kitchen pap r , ��Gcooled to room tem"�U��+vVB {��/ִn�deep-fat friu� at 180°C f���3 minu��s. T|V.1: Percentageٙ�rylamide �}otent u��potato cipsT(produced 1��w�potato tubers dirct��after harvest). Each �}rresponding wild ��]� was se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="29mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="24mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="26mm"/> <�}lspec colnum="4" colnam�Ǻcol4" colwidth="20mm"/> <�}lspec colnum="5" colnam�Ǻcol5" colwidth="26mm"/> <�}lspec colnum="6" colnam�Ǻcol6" colwidth="19mm"/> <�}lspec colnum="7" colnam�Ǻcol7" colwidth="26mm"/> Wild ��]� Tronsa 093IR360 Wild ��]� Solara 095IR049 Wild ��]� Bintje 092IR002 A�rylamide �}otent [%] 17% 62% 17% 56% 17% 64% The �bsolu^e ��rylamide �}otents �f [�C�potato cipsTproduced ��pdMc/vary �}osider|Vly_0etwQj�pth���ultiv+*s used. Th��p��pprimarily_due_to the differing �bsolu^e values m��solu���/sug+*s. Fz��instance potato cipsT1��w�cultiv+* Solara, ����example�GexhibitedB9r��mnly_/ִ�highest a�rylamide �}otents bu/aH9l�/ִ�highest solu���/sug+* �}otents. The relativɨ��rylamide �}otents �f [�C�potato cips, howev r, Xh�ed Cz��qll transgenic �ultiv+*s used a coosider|Vle reducti�j�in th��amount m����rylamide 0���pproximate��40-50% compared with potato cipsTw�5��� d �Qj�pproduced 1��w�wild ��]� tubers. In a further�analysis, th���}otents of solu���/sug+*s�G��pdMc�`�ar_�lucose,_fr�zose and�surose, were de�Rmined in the potato �ubers from the various �ultiv+*s: F���this purpose the potato �ubers�were peel�K=in a�cordance with example 2Ta��Gusing a �}rk borer (1��w�Roth),Ga�sample m��diame�R approximate��0.5 mm was �ut out. F��w�this �}rk borer sample�Ga��wpproximate��2 mw�thick slice was *akex in each �a�e ���w�the stdMc,�oneGquart r a۽�halfoway, 1��w�each m��5�different tubers , ��Gcombin�K=in a reaction vesse�Ta��Gused f���de�Rmining solu���/sug+*s. The �}otents of sug+*s�G��pdMc�`�ar_fr�zose and�lucose,_m��p��nt ����Ui&� were de�Rmined aspdescribed �bove. If th��totl �lucose �nd/���fr�zose �}otent i��correl���dBwith the ��rylamide �}otent in potato cips, it �€0e XQj�pthat �here > ���line+* �}rrel����j�0etwQj�pth���lucose �nd/���fr�zose �}otent and�the f������� 9f ��rylamide in the potato cips. ComparativɨExample 6 De�Rmination of the ��rylamide �}otent u��potato cipsTproduced 1��w�stor�dBpotato tubers m��differing vari�tDes�m��reduc�K=R1�-���,� X"Ic�O��j T|Vle�1, Percentageٙ�rylamide �}otent in potato cipsT(produced 1��w�potato tubers stor�dB9��4°C). The corresponding respe���b'$wild ��]�s were se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="29mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="24mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="26mm"/> <�}lspec colnum="4" colnam�Ǻcol4" colwidth="20mm"/> <�}lspec colnum="5" colnam�Ǻcol5" colwidth="26mm"/> <�}lspec colnum="6" colnam�Ǻcol6" colwidth="19mm"/> <�}lspec colnum="7" colnam�Ǻcol7" colwidth="26mm"/> Wild ��]� Tronsa 093IR360 Wild ��]� Solara 095IR049 Wild ��]� Bintje 092IR002 A�rylamide �}otent [%] 17% 55% 17% 70% 17% 58% The �bsolu^e ��rylamide �}otent in [�C�products from potatoesGstor�dB9��4°C always increases marked��. Howev r, it is found ����the vari�tDes�listed :ere,/aH9l, that �he ��rylamide �}otent in the potato cipsT1��w�/ransgenic potato tubers increases 0���pproximate��30-45% lesso�}mpared with the potato cipsT1��w�c}rresponding wild ��]� <��nts. In a further�exp�Uiment,�potato tubers m��/ִ�cultiv+* Désirée having reduc�K=R1-��,� X"Ic�O��j�(VLs:�OS�n9S���6789�����]�Ǻsi��T�E��Lorb��th e���H.,�o'�ure Biotechnology 16, (1998), 473-477) ere stor�dB9��4°C ����73 days. Potato cipsT, ��Gcrisps &ere produced aspdescribed in Example 1. Crisps &ere deep-fat friu� ����3 minu��s at 180°C aspdescribed in Example 2. In contr�st_to the potato crisps,�potato cipsTwere deep-fat pre-friu� at 180°C f���30 seconds, placed on kitchen pap r , ��Gcooled to room tem"�U��+vVB {��/ִn�deep-fat friu� f���3 minu��s. The potato cipsT, ��Gcrisps produced � d /ִ�f� towing �crylamide �}otents: T|Vle�2: Percentageٙ�rylamide �}otent u��potato cipsT, ��Gcrisps (produced 1��w�tubers stor�dB9��4°C). The wild ��]� was se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="58mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="55mm"/> <�}lspec colnum="3" colnam�Ǻcol3" colwidth="53mm"/> Potato cips Crisps deep-fat frying time deep-fat frying time 3 Oin 3 Oin Wild ��]� 17% 17% 009VL045 56% 33% The �bsolu^e ��rylamide �}otent in [�C�products from potatoesGstor�dB9��4°C always increases marked��. Howev r, it is aH9l�found in th��pexp�Uiment that �he ��rylamide �}otent increases 0���pproximate��70% lessoin the crisps made 1��w�/ransgenic potato tubers �}mpared with the �risps made 1��w�the �}rresponding wild ��]� <��nts. In a further�exp�Uiment,�potato tubers m��cultiv+* Désirée m��reduc�K=R1-��,� X"Ic�O��j�(VLs:�OS�n9S���6790�����]�Ǻsi��T�E��Lorb��th e���H.,�o'�ure Biotechnology 16, (1998), 473-477) ere stor�dB9��8°C ����73 days. These�stor�dBpotato tubers were processedB�nto potato cips in a�cordance with Example 1 a۽�deep-fat pre-friu� f���30 seconds�at 180°C�in a�cordance with Example 2, placed on kitchen pap r , ��Gcooled to room tem"�U��+vVB {��/ִn�deep-fat friu� at 180°C f���3 minu��s. T|Vle�3: Percentageٙ�rylamide �}otent u��potato cipsT(produced 1��w�tubers stor�dB9��8°C). The wild ��]� was se���ta100%. <�}lspec colnum="1" colnam�Ǻcol1" colwidth="84mm"/> <�}lspec colnum="2" colnam�Ǻcol2" colwidth="82mm"/> Potato cips deep-fat frying time 3 Oin Wild ��]� 17% 009VL045 52% The �bsolu^e ��rylamide �}otent in [�C�products from potatoBtuber stor�dB9��8°C doesG9r��:wcrease�as gre� asoin the ca�e m��potato tubers stor�dB9��4°C. Howev r, in th��pexp�Uiment aH9l, it is found that �he ��rylamide �}otent in the potato cipsT1��w�/ransgenic potato tubers increases 0���pproximate��48% lesso�}mpared with potato cipsTmade 1��w��}rresponding wild ��]� <��nts. <�laims id="�laims19�lang="en"> <�laim id="�-en-01-6719�num="6019> <�laim-text>P6cessof���reducing the a�rylamide �}otent u��heat-tre���dB��ods compared with corresponding conventӗd&�Theat-tre���dB��ods comprising: <�laim-text>(a) sel{�ing ��,�tDe��- modified <��nt ����Ui&�mrigi�aing from potatoB<��nts, w:ereinpth���en3tDeDmodification�leads to a reduction in th���}otent of solu���/sug+*s, compared with corresponding non-��,�tDe��- modified <��nt ����Ui&�1��w�wild ��]� <��nts, a۽�w:ereinps�id��en3tDeDmodification�leads to a reduction in th��q���b�?��u��oneGor mXre endo�enous R1 proteins mccurring in the p��nt cell �}mpared with corresponding p��nt cells�m��wild ��]� <��nts &�5��� ve 9r��Qj�p��,�tDe��- modified, <�laim-text>(b) processing the s�id�<��nt ����Ui&�to givɨ�B��od, a۽ <�laim-text>(c)Theat-tre��ing the ��odTproduced ��p6cessostep b), w:ereinpth����odTis heat �re���dBby grilling, roasting�Gextruding�Gbaking or microw ve he��ing. <�laim id="�-en-01-672" num="6029> <�laim-text>P6cessoaccordy�g_to Claim 1, in &�5��the s�id���rylamide �}otent is reduc�K=0����least 15% compared with the ��rylamide �}otent u��corresponding conventӗd&�Theat-tre���dB��ods. <�laim id="�-en-01-673" num="60039> <�laim-text>P6cessoaccordy�g_to Claim 1, in &�5��the s�id���rylamide �}otent is reduc�K=0����least 30% compared with the ��rylamide �}otent u��corresponding conventӗd&�Theat-tre���dB��ods. <�laim id="�-en-01-674" num="604"> <�laim-text>P6cessoaccordy�g_to o,� m��Claims 1_to 3, in &�5��the s�id�heat �re��ment i��carriu� out at tem"�U��+vVs m��a�least 100°C. <�laim id="�-en-01-67�9��d�d���5"> <�laim-text>P6cessoaccordy�g_to o,� m��[�C�prec�King claims, in &�5��the s�id��en3tDeDmodification���pth���ntroducti�j�m��oneGor mXre X�eign��dcleDeDwci�GXlecul�s,�the pvVsence �nd/���X"Ic�O��j�m��&�5��leads to the reduction in th��q���b�?��u��oneGor mXre endo�enous R1 proteins mccurring in the p��nt cell �}mpared with corresponding p��nt cells�m��wild ��]� <��nts &�5��� ve 9r��Qj�p��,�tDe��- modified. <�laim id="�-en-01-6769��d�d���6�k <�laim-text>P6cessoaccordy�g_to Claim 5, in &�5��the s�id�X�eign��dcleDeDwci�GXlecul�s�are sel{�ed 1��w�the group��}nsisting�m� <�laim-text>(a) ٽ�<Xlecul�s�:�o�}de f���a�least one�w�tisense R��<�ausing a reduction in X"Ic�O��j�m��endo�enous ��,�s :�o�}de f���R1 proteins, <�laim-text>(b) ٽ�<Xlecul�s�:�, via a cosup"Ic�O��j�effec�,�lead to reduction of the X"Ic�O��j�m��endo�enous ��,�s �}dy�g_f���R1 proteins, <�laim-text>(c) ٽ�<Xlecul�s�:�o�}de f���a�least one�ribozy�e:�o�leaves in a sk}kf�eDmanneR�transcripts�m��endo�enous ��,�s �}dy�g_f���R1 proteins, <�laim-text>(d)��dcleDeDwci�GXlecul�s�&�5��are �ntroduc�K=0��means <�laim-text>(e) ٽ�<Xlecul�s�:�osiU��taneouslyo�}de f���a�least one�w�tisense R�� <�laim-text>(f) ٽ�<Xlecul�s�:�o�}otain transposons, the :wtegration of the transposon sequences leady�g_to a mutation�mr�an�inser��� in Xndo�enous ��,�s �}dy�g_f���R1 proteins:�o��=�es�a reduction in the X"Ic�O��j�m��the s�id��en3s mr the syn�hesis m��ina���b'$R1 proteins, a۽ <�laim-text>(g) T-ٽ�<Xlecul�s�:�, via inser��� in Xndo�enous ��,�s �}dy�g_f���R1 proteino��=�e�a reduction in the X"Ic�O��j�m����,�s �}dy�g_f���R1 proteinomr the syn�hesis m��ina���b'$R1 proteins. <�laim id="�-en-01-6779��d�d���079> <�laim-text>P6cessoaccordy�g_to o,� m���laims 1-6, in &�5��the s�id�heat-tre���dB��ods are sel{�ed 1��w�the group��}nsisting�m�_potato cips, potato crisps,�parfriu� potato cips, mash�K=potato a۽�potato sticks. <�laims id="�laims29�lang="de"> <�laim id="�-de-01-6719�num="6019> <�laim-text>Verfahrj�pzur Verringerung des A�rylamidgehal��s v� ärmebehand�l��n Lebensmitteln im VergleDehpzu entsprechXnden herkömmlichen ärmebehand�l��n Lebensmitteln umfass jd: <�laim-text>(a) die Auswahl v� ��,�tDsomXdifizier�em pflanzlichen M���Ui&�, das aus Kartoffelpflanzj�pXtamm�,�wobei die ��,�tDse MXdifikation�zu eineR�Verringerung des Gehal��s an�löslichen Zucke*n führt im VergleDehpzu entsprechXndem nDeht ��,�tDsomXdifizier�en pflanzlichen M���Ui&� v� Wild��]-Pflanzj�, =nd�wobei die besagte ��,�tDse MXdifikation�zu eineR�Verringerung der Ak��b�?ät eines mder mehrjr�r in der Pflanzj�zelle v�rkommend�� Xndo�ene��R1-Proteine führt im VergleDehpzu entsprechXnden nDeht ��,�tDsomXdifizier�en Pflanzj�zellen v� Wild��]-Pflanzj�, <�laim-text>(b) die Verarb�itung des besagten pflanzlichen M���Ui&�s�zu einem Lebensmittel, =nd <�laim-text>(c) die Wärmebehandlung des in Verfahrj�ssritt b) erzeug��n Lebensmittels, wobei das Lebensmittel duRoGrillj�, Brate�, Extrudieren, Backex mder Mikrowellenerärmung ärmebehand�l�wird. <�laim id="�-de-01-672" num="6029> <�laim-text>Verfahrj�pnach Anspruo1, &orin besagter A�rylamidgehal� um Oindestens15% verringert i�t im VergleDehpzum A�rylamidgehal� v� entsprechXnden herkömmlichen ärmebehand�l��n Lebensmitteln. <�laim id="�-de-01-673" num="60039> <�laim-text>Verfahrj�pnach Anspruo1, &orin besagter A�rylamidgehal� um Oindestens30% verringert i�t im VergleDehpzum A�rylamidgehal� v� entsprechXnden herkömmlichen ärmebehand�l��n Lebensmitteln. <�laim id="�-de-01-674" num="604"> <�laim-text>Verfahrj�pnach einem der Ansprüe 1 bis 3, &orin die besagte Wärmebehandlung bei Tem"�U��+vVn v� Oindestens100°C erf�lgt. <�laim id="�-de-01-67�9��d�d���5"> <�laim-text>Verfahrj�pnach einem der v�rangehXnden Ansprüe, &orin die besagte ��,�tDse MXdifikation�die Einführung eines mder mehrjr�r fremder Ndcle�nsä+vVXleküle > �,�deren Vorhand�nsein und/�der E"Ic�O��j�zur Verringerung der Ak��b�?ät eines mder mehrjr�r in der Pflanzj�zelle v�rkommend�� Xndo�ene��R1-Proteine führt im VergleDehpzu entsprechXnden nDeht ��,�tDsomXdifizier�en Pflanzj�zellen v� Wild��]-Pflanzj�. <�laim id="�-de-01-6769��d�d���6�k <�laim-text>Verfahrj�pnach Anspruo5, &orin die besagten fremden Ndcle�nsä+vVXleküle ausgeähl� sind aus d�� Gruppe bestehXnd aus <�laim-text>(a) ٽ�-MXlekülen, die Oindestenseine ۜtisense-R��<�odieren, wele eine Verringerung der E"Ic�O��j�v� endo�enen Genen bewirkt, die R1-Proteine �odieren, <�laim-text>(b) ٽ�-MXlekülen, die über einen Cosup"Ic�O��jseffekt�zur Verringerung der E"Ic�O��j�v� endo�enen Genen führen, die R1-Proteine �odieren, <�laim-text>(c) ٽ�-MXlekülen, die Oindestensein Ribozy�<�odieren, das spezifisoTranskripte�v� endo�enen Genen spal��t, die R1-Proteine �odieren, <�laim-text>(d) Ndcle�nsä+vVXlekülen, die Oittels�in vivo-Mutagenese eingeführt werden =nd�die zu eineR�Mutation�mder einer Inser��� einer he��Uologen Sequenz in Xndo�enen R1-Proteinen �odierenden Genen führen, wobei die Mutation�mder Inser��� eine Verringerung der E"Ic�O��j�besagter G�,� mder die Syn�hese�v� inak��ben R1-Proteinen bewirkt, <�laim-text>(e) ٽ�-MXlekülen, die siU��tan Oindestenseine ۜtisense-R��<=nd�Oindestenseine sense-R��<�odieren, wobei die besagte ۜtisense-R��<=nd�besagte sense-R�� <�laim-text>(f) ٽ�-MXlekülen, die Transposons enthal��n, wobei die Iwtegration der Transposonsequenzen zu eineR�Mutation�mder einer Inser��� in R1-Proteinen �odierenden endo�enen Genen führ�,�was eine Verringerung der E"Ic�O��j�besagter G�,� mder die Syn�hese�v� inak��ben R1-Proteinen bewirkt, =nd <�laim-text>(g) T-ٽ� <�laim id="�-de-01-6779��d�d���079> <�laim-text>Verfahrj�pnach einem der Ansprüe 1-6, &orin die besagten ärmebehand�l��n Lebensmittel ausgeähl� sind aus d�� Gruppe bestehXnd aus Pommes Frit�s,�Kartoffelcips, v�rfrittier�en Pommes Frit�s,�Kartoffelbrei, =nd�Kartoffelsticks. <�laims id="�laims39�lang="fr"> <�laim id="�-fr-01-6719�num="6019> <�laim-text>P6cédé de réduction de la �eneu� Xn ��rylamide d'aliments ay�nt subi =n trait�ment thRmique,�par �}mparaison avec des aliments �la�O�ques correspondants ay�nt subi =n trait�ment thRmique,�compren�nt <�laim-text>(a) la sél{�ion d'=n matériel végétl �énétiquement mXdifié, proven�nt de <��nts de <�laim-text>(b) le trait�ment dudit ��tériel végétl pou� ob�enir =n aliment , et <�laim-text>(c) le trait�ment thRmique de l'qliment produit dans l'qliment étnt traité thRmiquement par_�rillade,�rôtiss�ge, extruO��j,�cuisson au four ou chauffageٙux micro-ondes. <�laim id="�-fr-01-672" num="6029> <�laim-text>P6cédé selon�la revendication�1, dans <�laim id="�-fr-01-673" num="60039> <�laim-text>P6cédé selon�la revendication�1, dans --> <�laim id="�-fr-01-674" num="604"> <�laim-text>P6cédé selon�l'=ne des revendications 1_à 3, dans <�laim id="�-fr-01-67�9��d�d���5"> <�laim-text>P6cédé selon�l'=ne des revendications précédents, dans <�laim id="�-fr-01-6769��d�d���6�k <�laim-text>P6cédé selon�la revendication�5, dans(a) d�sTXlécul�sd'ADN qui �}dent pour au mXins=n ARN ۜtisens, provoqu�nt =ne réduction de l'X"Ic�O��j�de gène endo�ènes qui �}dent pour desTpro?éines R1 , <�laim-text>(b) d�sTXlécul�sd'ADN qui,�par l'�ntRmédiaire d'=n effet de cosup"Ic�O��j,��}o�uisent à =ne réduction de l'X"Ic�O��j�de gènes endo�ènes �}dant pour desTpro?éines R1 , <�laim-text>(c) desTpro?éines d'ADN qui �}dent pour au mXins=n ribozy�e, qui d'=ne manière s"ékf�que��l�bent desTpro�uits de �ranscriptӗd de gènes endo�ènes �}dant pour desTpro?éines R1 , <�laim-text>(d) d�sTXlécul�sd'acides��dcléiques qui sont �ntroduit�sTau mXyej�d'=ne mutagenèse in vivo et qui �}o�uisent à =ne mutation�mu à =ne inser��� d'=ne séquence hétéUologue dax�pdes �ènes �}dant pour desTpro?éines R1 endo�ènes, l�Dmutation�mu l'�nser��� provoqu�nt =ne réduction de l'X"Ic�O��j�desdits �ènes mu de la syn�hèse deTpro?éines R1 ina���b's , <�laim-text>(e) d�sTXlécul�sd'ADN qui siU��tanément codent pour au mXins=n ARN ۜtisens et pour au mXins=n ARN sens, ledit ARN ۜtisens et ledit ARN sens f����nt =ne Xlécul�d'ARN dou��� brin, qui provoque =ne réduction de l'X"Ic�O��j�de gènes endo�ènes �}dant pour desTpro?éines R1 , <�laim-text>(f) d�sTXlécul�sd'ADN qui �}�tiennent desTtransposons, l'�ntégration des séquences de �ransposons �}o�uis�nt à =ne mutation�mu à =ne inser��� dax�pdes �ènes endo�ènes �}dant pour desTpro?éines R1,��e qui provoque =ne réduction de l'X"Ic�O��j�desdits �ènes mu de la syn�hèse deTpro?éines R1 ina���b's , et <�laim-text>(g) d�sTXlécul�sd'ADN-T qui,�par l'�ntRmédiaire d'=ne inser��� dax�pdes �ènes endo�ènes �}dant pour =ne pro?éine RI, provoquent =ne réduction de l'X"Ic�O��j�de gènes �}dant pour =ne pro?éine R1 mu de la syn�hèse deTpro?éines R1 ina���b's. <�laim id="�-fr-01-6779��d�d���079> <�laim-text>P6cédé selon�l'=ne des revendications 1_à 6, dans REFERENCES CITED IN THE DESCRIPTION Th��pli�t m��references Patent documents WO9740707A1[719] WO9827212A[735] WO6777229A[735] WO6728052A[735] WO9704112A[750] WO9704113A[750] WO9837213A[750] WO9837214A[750] EP6321201B1[752] WO9515972A[753] WO995350A1[755] WO9711188A[777][783] Non-patent lit�U��+vVBcited in the descriptӗd FAO/WHO C�js��tation�mn the Health ImplicationsWHO2720625[702][709][729] MOTTRAM 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EXTENDED RANGE SINGLE-JOINT ELEVATOR EP17167413.8
[ANGELLE��j?���A%-5�{��sj��9�oM{[�}��O�A�~_BH�{���[��#�A⫧q���M�,�s�@#*w����!�b���x\(��^ۛ �I%,��U�)��|�Ka/~���"_*,=1@ڭ���)��ـ���թ�b})�V�@!tm��� r��n����Zڅc��Y����e�\@9ϕ�Y���d"�_m���i?�C������J����� ���(�m7���so�m�3���<�&*�,�k��e���Y������������5q�]/������"My4$�դ�ޅ��HGY ��Cq���↝�~����s ?Sm��f���������ȿ}d�衢���g�=#&���I�]9t ��A,�"�,���Y(a1( q��� ,�6���錥F%��>��WL��\�X3Q��\��rfy'D'7�7n''K+-7�/��r��L1!����������0́{O �21>] US,Houston, TX 77042,10260 Westheimer, Suite 700; An elevator (1100) to manipulate a tub��h-C����g�-� >�ޒ�5��>����<������� #�}��F�m�0$$.%�Зi�Vw��Жp���Z��(�/a-9(��Ȭ�5��]p^��n�@X�7��X���jw�^f�3��>�_"�j���y���1��9�2,d�)��[L���S|�8��(^|*|=n�4�t�4d�%�<���4�4y��z������C��=i̦�&[W����7��'��6�G�ߔ�1���m�X�,Z-J.�y��w&j�^�� �K�<�u�u-�Jt���?/k�(���42I��Z�K;_�Ϲs�q�w�r�p���5}S��������R2�~��g�dpCtֆ� �5�9کM�z���jf`���pQ��#�[�f��d^K�y�pv�9׫?xP��qT3\n�b����`����� � 5�[�/���jtE}���������;(5&�'9dž��v��n � i �����§z}1�e�(5(� C�(z\z�t9�;m�l���K+P inside the�plurality of openings�d�coupled to�the�R��oL��P��=�-xeachof the�plurality of slip assembs��s�r�E��ing]��actuoL��P��=�B8\21)�coupled to�the�R��oL��P��=�-xa slip B8\24)-xthe�slip �r�E��ing]��engage��ꐵe�S��3B8\26��Hn¹+�B� gN�hogonal to�the��R֣�of the�ɮ�\�)2�����:Yʠ��(Y�ӛA���tDengage��mقL�l#�%H:����.
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METHOD FOR MANGLING ITEMS OF LAUNDRY AND MANGLE SECTION EP17000142.4
[Engel ^� �3���)�S‚�F��0�<{�\(%�,[~~z�qrp�)�V��5��{'d�ǃ��@R����?�j���q�N}r��HI��_�٦ꭚ@ �:��������S�=���]�--�r��Z�|7vw"�ʯ�~adR�u皶ةG��S��Q��H��ϸ�?DM�i�@k, �%�ŸP7��_,�۩O��h��8�C���1iˠ���y��H��es� �x�iqW�g��+�_G$=��uD��l�C�T g�� zr�j�pt�x 1щ��,N��]��|7m��������� �p�W�a��U�^�Ip��)���G� I�L"Tw�uû7D>l���i�b�1t��HQA�f���T>͇ /�R���y�!�E|��7�)�I��1�'t��^�y>ATBEBGCHCYCZDEDKEEESFIFRGBGRHRHUIEISITLILTLULVMCMKMTNLNOPLPTRORSSESISKSMTRBAMEMAMD Werden WäG�N��ücke in Muldenmangeln (11) od�.X�änd�.mangeln (12) gemangel��wird die erforder� h*]a�8�m�Z$�tT&�c�q��Ό�1��f��v�ڨ�, .5����(iJ�����F�HF������l���� ����fdޜ.��m�X��8s �A0�na����d�[F\A��&���)�W��pqi`��I\"�ܰ�Q��$W9|9EY9j��ӌl���XD_W�v�u����Uk}��A�:�|�V�c�D���iUp�p+ڔ�3l�(��d𔸃w>j|k��(k|E�Z�$��-� ��T��滚�$��U�m�/�bz��, �����c=��G�^ݗ��*\̓� �g��8�L�\�������ۥ��s 4�r�?���ѡ<�jrh �U���L)m@U�}"g#��g�_SUmȉ�/�L �>��~h�]m��x�Ž����h��_C��Y ���j�t�M�R�G}�� �T[���ر-��3�1���C:�`���� �� Z��O��p�G��und�dk�P�änd�.mangel (12)�furch Plättflä�N�n (18, 28) an ge":über�e":�Z�S��n Wärme zu/��E�~���urch steigҖϋ�4�����39���ٟundaϣ*Wpa���͐`3�so�1ޒZ�S��n d�.XWäG�N��ücke ,ř"���� ��:��/ab�s%Qct:��description��P[[desc" lang="de">] DE,32602 Vlotho,Kannegiesserring 7;
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Image forming apparatus that indicates operating status using luminous body EP13162208.6
[Tomo|�F�����kU&P?����4m�< �fs����`W���t� ���>�o������Ҷ�p���ɗ�s�� �����靄�t��d�{���w����gj�mPD9d�8�<=� ,(�ϭ��G��A^O����A���~0�p�,|�?�G�GkqG�F�]���L ��\��[xM�)y�����퓬E��+Q����ۀ�E˒B� �k Y��l%].�C zW�6}���, �@�k�pc�>���"�Od���ƣ��͞\_:3�O���)���Գ��y!�iS�����ʺ���M[�Q��CN�>ESFIFRGBGRHRHUIEISI�ZH� e�'��{��LIL�ZH� e�'��{��LULVMCMw� ���G�PM�ZH� e�'��{��N�D����a9Ǿ]NOD����a9Ǿ]P�D����a9Ǿ]P�ZH� e�'��{��ROD����a9Ǿ]RSSESISw� ���G�PSM� ���G�PTR<�Q���A<�Q��ME20171011201741 An image forming apparatus includes an image forming section, a body housing, a protruding housing, a light diffusing portion, a light source, a light guide path, and a control unit. The image forming section performs image formation on a sheet. The body housing is formed by a box body with a top surface, and contains the image forming section. The protruding housing is located on the top surface, and has a top portion protruding upwardly above the top surface. The light diffusing portion is located in or near the top portion of the protruding housing. The light source is located inside the body housing or the protruding housing�&�K�, h4 k�,7Xo�`QR��z����V���^r�# �R �k-R%��� g�K~��j2��Z���d@�z�+m�e�^�vˀ�n�zõ�Zgr�H+~�b���I?�Сq�C}S5�]��T,�V�*j��������ZS7bΘ�J��z�T��۹3-Ϻ�噷���N� �6y+�l�g���ۗ���ߺ>�'�Xi�65�m 囱}��x�HF���9T9�Һ3s (k��lz��ٌ*�n%7��$�ile="sre0002.tif" wi="155" he="233" type="tif"/"�/search-re"(�-data> �W-re-�1p�>EP13162208.6<��<�@b dnum="JP2000280�A" id="sr-p�@b0001" url="http://v3.espacenet.com���<]?DB=EPODOC&IDX=JP2000280�&CY=��L֚^xument-�b�JP2000280�A�nameLCANON KK?���001010�������ocument-�b�X<���-claims>1-�����-claims><���-po�Xi"�><�ͱXi"�>* paragraphs [�003], [�025], [�026], [�030], Fh�N�s 1,4 *�nameLM greoli, Loenzo�/examinerECq&f���v��~o/%+[�?�Ki鞫aU$���v��R�ch[Dw�� {���4�q�r�`����m����箯�F)XML��yǖ��comple(gG �>?���C830�������Zi�-*8��O��comple(gG /-re-a�9h��!-- ���{annex-��sts the patent family membersٍ��{��"G���$���patent ocuments �@bed in $���above mentioned European search re"(�. ���{members�ar��as contained in $���European Rd O�箯 EDP�tile on ���{European Rd O�箯 is in no waX� ��$���|��))�iۿ�O�ʽ�܆u���}T����}=�\9��]�_X�K�ѹ,x�)�}�06Qo����B� ֫��9=����u��z��y�-�� ��ň_ _/_>#���z, ���q��T�G+}��a�!�-:y�#Mc�K���Rz�|, p��Բ�u���df�έ�3�{|���?���>0�l{ �Xf�"��=�ٜ#�I�?�uт�S)�,F��{,#������onL֚^xument-�b�JP2000280�A��>?���001010�������ocument-�b�0�l{ �Xf�"��=/�|��j�›���rR�/search-re"(�-data>] JP,Chuo-ku Osaka 540-8585,2-28, 1-chome Tamatsukuri;
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DETECTION CHIP AND DETECTION METHOD EP15868386.2
[NAGAE, Kosu�(��$(, Ŗ��ߏ미�b��n+N�GK��f��, L��j���g���s����M&ڌ=��u��H���֮�ZU!��k�������e�ڿ����ց޷��lG�����/\�L0u3 ���gwއ��%�kƷ�_��G�s���� ��^�� ��טŶ��Ȅ�Fh)6B��y�3��nv���.����}�����醝U��"�JPKW�3i%}1!�S��^�g��D����FUI���a�g����א��xi�if��]������K����}=oIwy��?ۡ�%B����c�H�D��4�eרF����+�j������XIp�1�\�bHQ_Z�[�o����~q4�O��J�ƶ�, ��n|N����)���SV&���J��"2�:L�Vy��P�U�O����������@��M�]��5& ��@*���i�z\�g�Z��u�$��Y�U� ����t��$�lY%�Y��3�g�j���&��.��(�u�|F��_� #�r>Brucknerstrasse 2040593 DüsseldorfDE] JP,Tokyo 100-7015,2-7-2 Marunouchi Chiyoda-ku; The detection =k2p according to the present invention has an accommVoting part, a metal film, a first reaction field, and a second reaction field. The accommVoting part accommVotes a liquid. The metal film is ar�=�M"��t.k�n�CG�ԕ<[��H���������n� "5[���Ή��6|��V��ͅ=&��O�7�� �^���L���Zx�_s�B(��O�����~=Xz(pd�� ��%��e^UpE�@��^.n�wA� .W|i6��JH��v8� B�c�4�\�cz yگ爴u6�� !���z��i������3+�o*E��_�:U98�&��^�A���C4U�E��9�W��42�vs�bCyݱvt���}�=��K嗣���j�Xԕ� C�U�L��?��� �$��?�����H�EgA�>�+oa�=xSP�aQp���ä�M��O�b���J:8!/<�'gط.8�0� �\� �!��E�`L��y;&B:�e���?� �|���~??���bbВ.6���Yܫ9�HêZ~�2�ۧ �sUQ���>tZ���ʩO�9w�b>��>k D]�LL�5`j&��MQ&�Z6�����y��z����0M�q ��08�V~W�x�eA�="iaf01" src="${rootURL}/view/EP3232182A1/20171018/imgaf001.tif" width="102" height="82" img-content="drawing" img-format="tif"/>
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Sensor for detection of wrapping on bale. EP10161805.6
[Smith, Kevin M., Foster, Christopher A., � �����xRή��-{��[�����"B"�O��, ��*��ue4-�elݟ>� �c_�K�陕��J���qkت�9/։x�-7YU�1ش3$(�=w�3���w�f4 ��kW�T��0Xrjޭ�t�>��KnuςcW �9����/m�y���y��Z�e./e�Z�r\O��Y�pO�KgE��|J������e�I�3k��� ����r�� ��କV��'�ž?�+#����7�3�Ȟ��bo���� DŽ1�<�V{<�?-����*�ʙv�S��pK���2[�#��1�@!,�r�� ���D�K�E���.R/2�2<�� j�ȏя=�!Te�!�\��.LU�H�’2�À�]��z���פƒ,Snh�G'�喤wc_f��Ns8L�{�S��b `�)#�!h����# �.�FOB�u�n�Q_��Z��\3UɄ�, Bص�l�������������!�C4�����i<�k�"��u����K���UR�2Bߙtry>FIFRGBGRHRHUIEISITLILTLULVMCMKMTNLNOPLPTROSESISKSMTRBAMERS20171011201741] BE,8210 Zedelgem,Leon Claeysstraat 3A; A baling machine and method for forming a bale is disclosed. A sensor 82 is positioned in the baling chamber and is positioned to sense a region proximate the perimeter of the bale to determine if mate���ބis present, thereby determining if the bale is properly wrapped or not. When the bale is not properly wrapped, mate���ބis present in the region proximate the perimeter in which the sensor is active, causing the sensor to send a sign�ބindicating th��.the bale is not properly wrapped. �1qinfo> EP 10161805.6 1 �1qinfo-admin>�1q�{�y��.*�dressbook> DH 20170908 /�1qinfo><�1qfor-pubd��1qfields���tS}KJd> A01F <�1q{�ations�E�Eation�>c4�q{�0001">
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Compact high-pressure exhaust muffling devices EP12196992.7
[Masonr��ij�� �ApD��˝!X' ����:/8� �JcKcu䝍�������ê������|u[�~U���-lLQRT$Hʬ2+�E|�M �P/v\-�qZ�&x��j��jT�D$o�0�Y�j���t��=?Kh���?� oaRG}}�\pJk�&ҧ��ew'�[��q\�g\�y�Ԝ��9��k��Xh�6�dGH�J��EIE�*S�:� lqr�wb���ߏ����ӭ�� *�>��1�9�}����+�6L�����k$~������#��8�Uw����V馩^�� ���O9���*L��$A�@�����=s�v��}p֩��X`��, ����۲v���ȥ^t=�������o�WW���.�]~����ְ������7 �gm�p��TZp�rv�L�%Gz=�}��b#s��\}t�ջ%5&xR�J �g��胭Ŗ����E�{?OK��ܣ�9�Z���=�, �i�֪��c�[�N�tIj�ؓb"�:ޗSR�ɱ V�nm>Lawless, Charles Steve] US,Schenectady, NY 12345,1 River Road; Compact, high-pressure exhaust muffling devices (50) are discq8ed. Some example muffling devices (50) may include an in��; flow condition�; (110) including an inlet (118) configured to֣�Px��m &t�4c!1��!�հ��Őd����w?4W�宍�˓vml�0�U�LA��c�s��|�f�Me��+������y�K��,�ꜳ��q�z���'%�[����۩����Mnɟ���s��]��'�����o�W�[��"߲e4 �,4��#�"�c�(���^!%��n0dD#�$�+X�����o��*[�̤���)�c���S����ү~7?:k4�J�}ad���;��P��$d�8h�%�)�:J�>�\�ˍ%�Y|J�vlq���� '�"�k[.�s^VGTo;�3��A��7�[�^u$+Y/;��"����t����#Φ��Ф��9��`�<�LK��L��:�n�1/a�C&k�K�q��=RB�R!,��;*!E�x�AؔaϮ�+=�OcQ��`���'�W,ԧ_�O椯ȱQ� �j'�+�>���پ)�D�}��W>���q6�Ƣe���Q5g��Ns��ײ1�^�.����0�)��E�i�c�`_ɦ/�arranged to discharge the pressurized fluid flow from the exhaust can interio; (108). The exhaust can interio; (108) may be substantiall��devoid of flow obstructions between the plurality of in��; flow condition�; holes (120, 122) S���the exhaust screen holes (106).
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